Is there an open source tool the measures the GRAVY index of a peptide incorporating the PTMs and unusual amino acids (e.g. hydroxyproline)?

Right now, I am using ProtParam to compute GRAVY index of peptides. However, some of my peptides are post-translationally modified, and contains unusual amino acids. I assume that ProtParam does...

29 January 2021 8,300 4 View

How long does the peptide cleavage cocktail last before it gets expired?

The cleavage cocktail is composed of Phenol/thioanisole/water/TFA/triisopropylsilane. I only prepare 10 mL cocktail per peptide but can the activity of the cocktail prepared at 100 mL last for a week?

10 January 2021 7,424 3 View

Could you recommend an inexpensive camera for a prototype DIC system?

I was thinking of choosing Raspberry pi HQ 12MP. and what software do you recommend. I am working on a measurement system for a plate with an area of approx. 500x500 mm2

30 December 2020 3,064 2 View

Is there an interpretation for Lineweaver-Burk plot which linear regression lines intersect themselves on quadrant I?

Lineweaver-Burk replots for enzyme inhibition prints regression lines intersecting themselves on quadrant I. The interpretations for competitive, noncompetitive, or uncompetitive inhibition does...

24 November 2020 7,753 3 View

Protein long term storage can have an effect in pH changes?

I have a long term stability study with a protein that as an isoletric point lower than the buffer (Tris buffer), and I am seeing a sustantial drift from the initial pH to 12 month point....

10 November 2020 4,445 3 View

How is vertical agriculture progressing in Colombia?

Vertical agriculture offers an innovative solution to reduce the pressure of cultivation on soils. The intensive use of machinery and large areas of land harm biodiversity.

28 October 2020 8,773 3 View

When having a particular combination of two drugs in the same nanoparticle, how to discriminate between additive and synergistic anticancer activity?

Both additive and synergistic activities have been described for chemotherapy drug combinations co-loaded in the same nanoparticle. Such strategies have been aimed at cancer therapy via dual...

05 October 2020 5,027 5 View

How to crystallize curcumin from ethanolic turmeric extract?

I'm having difficulty recovering curcumin from my ethanolic extract. I tried reducing its solubility in the solution by slowly adding water, but the solid just crashed out of the solution in very...

03 September 2020 3,020 5 View

Recommend antibodies that works in paraffin sections for synaptic and astrocytes markers in rat samples?

Hello, I am trying to find antibodies that can be used in paraffin rat brain sections against S100B , PSD95, Synapsin-I, VGLUT1,VGLUT2. I am checking the technical info provided by some companies...

12 August 2020 5,382 2 View

Incorrect FEM stress analysis with quadrilateral element... why?

Hi everyone, To put this in context, , I am making a Matlab algorithm for topology optimization that consider fatigue breaking. I am basing on the Ole Sigmund algorithm, which discretices the 2D...

11 August 2020 1,532 6 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

What is the correct common name for Hydrochoerus hydrochaeris?

There are two extant species of Hydrochoerus: Hydrochoerus isthmius, the lesser or Panamanian capybara, and the genotype species H. hydrochaeris. The latter is the more common species of capybara,...

11 February 2021 3,494 14 View

Is there an alternative to PGLS that considers phylogenetic signal when calculating the estimated value?

I have been working on a study trying to estimate body mass in mammals using a skeletal proxy in R. My dataset has high phylogenetic signal (Pagel's Lambda = 0.9) and I am interested in removing...

26 January 2021 6,997 3 View

Is there any relevant technique for mesocosm studies in marine environment using Nanoparticles?

I am planning to perform mesocosm study in simulated marine environment using Phosphorus based nanoparticles, i would be using marine micro-organisms in it. kindly suggest me some effective...

07 January 2021 5,877 3 View

How does titanium dioxide (TiO2) as a coating's component enhance the coating performance in terms of anticorrosion??

Greetings, Recently, I was asked by a researcher how does TiO2 enhance the coatings polymeric structure making it able to reduce corrosion reaction by preventing the penetration of aggressive...

02 January 2021 5,257 5 View

Carboniferous shark teeth - ID help?

Dear everyone! I am looking for some help regarding the identification of two Carboniferous shark teeth from Hungary. See the pic attached. I'd go for Bransonellidae family for A, and Falcatidae...

21 December 2020 1,226 4 View

Is there any chemical safe for sea environment, but could be used for repelling planktons and microorganisms?

As an example of Julia Child, whose first receipe was shark repelent, my team and I are looking for some chemicals which wont harm planktons but will help keep planktons from our robot in sea.

20 December 2020 9,616 4 View

What is the most controllable (marine) bacterial culture you can cultivate in simulated seawater?

02 December 2020 6,796 3 View

Any available international research grant in this COVID-19 year?

Dear researchers, I am a researcher from a university in Malaysia. I know that we all are having a tough situation in this pandemic year. I just posted this to know if there is any possible...

24 November 2020 8,759 3 View