Both Ct and Cq values are used in gene expression studies. Is there any difference between these two values. If yes, when do we use Ct values and when Cq value?
Ct and Cq are the same. But the application method of this value could result in different results. There are multiple methods for qPCR data analysis focusing the inaccurate results due to perfect amplification assumption. Tranditionally 2-delta delta Ct method is used.