Reasons for caspases cleavage in control samples?

Hello, recently I have been detecting caspases cleavage with western blot. But I found caspases cleavage in control/untreated group, what can be the possible reasons? I used QGP-1 cell line. The...

05 June 2018 3,568 4 View

Is there any recomended open source for colony counting

Dear all, Have you ever used open colony counting software? Now i am using OpenCFU, but it broke down several time during analysis. Do you have better suggestions?

05 June 2017 3,731 1 View

What are differences among p-value adjustment methods in microarray?

Dear all, what are the differences among empirical bayes, fast t-test,t-test,mann-whitney,LPE and ramkprod? i have Treatment Group microarray mRNA intensity and non-Treatment Group. now i wanna...

02 March 2017 4,657 4 View

NAC induces ROS instead of supressing

Dear scientific fighters, Has anyone met this problem? NAC is supposed to be ROS scavenger. At beginning it worked fine, but later in my experiment, NAC increased ROS. pH is neutral. I used 20mM...

31 December 2016 4,047 5 View

Can anyone recommend some free software for creating heat map?

not geographic heat map. but can transform excel data to block heat map.

08 September 2016 3,871 1 View

Is the seeded cells really going through log phase?

For suspencious cells, we usually seed the cells to 96-well plate during the log Phase. But the procedure is just like passaging the cells. we Count the cells and then dilute the cells with fresh...

04 May 2016 5,193 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View