Is there any cheap method for protein desalting other than PD-10 Columns?

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Maurizio Francesco Brivio

conventional dyalisis with dyalisis tubes against large volumes of low salt buffer or milli Q water

Peter Yau

TCA precipitation sometimes work

Ahmed A. Abdelgalil

..Thanks for your suggestions ..

well, for detecting the presence or absence of advance glycation end products ..which of the above is good?

Pal Bela Szecsi

peptides may usually be precipitated in 10 volume cold diethyl ether.

small volume of proteins can be dialysed by placing a drop on a membrane (with low MW cut-off) floating on a beaker of water (or new buffer)