Do the extraction of dna will be as perfect or will it cause mutation if we inject it into a bacterial cell
Ethidium bromide is not the problem. But keep UV exposure low. Long irridiation may destroy your DNA. But if you keep exposure short you don't have to worry. Be aware that errors may occur during DNA amplification.
Ethidium bromide is not the problem. But keep UV exposure low. Long irridiation may destroy your DNA. But if you keep exposure short you don't have to worry. Be aware that errors may occur during DNA amplification.
Ethidium bromide (EB) is well known dye for DNA, it sticks to DNA irreversibly and makes it visual under Ultra violet (UV) waves. EB can cause mutation during DNA reproduction by sticking to DNA and make wrong nucleobase expression, so cannot make mutation during electrophoresis because no DNA production happens inside electrophoresis.
As Dr. Borgmeyer says you should worry about UV exposure not ED
As the other researchers stated it may damage if you expose it for long period. You can use alternative dyes like Midori Green, Safe Blue, SYBR green
I completely agree with the colleague that said that Ethidium bromide is not the problem. long UV exposure is the real problem because UV light could mutate or even destroy your DNA.
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