How to calculate contribution of each emission sector to atmospheric concentration of green house gasses?

If we measure atmospheric greenhouse gases for instance CH4 (methane) using CRDS instrument, how we can measure contribution emission CH4 from vehicle, rice paddy, wet land, farm etc, and how...

24 February 2020 3,535 3 View

Could NS-3 be used to simulate Analog Network Coding (ANC) or Physical-Layer Network Coding (PNC) in mesh wireless networks?

I am in a project to simulate how Analog Network Coding(ANC) or PNC works in Mesh Wireless Networks. Is it possible to simulate ANC or PNC in NS-3? I know there is KODO library for network coding...

20 February 2017 6,135 3 View

Is there any specific volume percentage (w/v) to buffer for lysing the bacterial pellet ?

Recently, i'm doing a sonication to bacterial pellet using PBS 10x buffer. I'm being confused in adding the buffer to the pellet for sonication. I'm setting the amplitude for 20-30%. Is there any...

27 October 2016 8,877 2 View

Why using the whole-cell biosensor gives a fluctuative signal when detecting AHL ?

I've been working with E.coli JM109 pSB401 for detecting the C8-HSL from Burkholderia cenocepacia species. The AHL was extracted using Acidified Ethyl-acetate and then suspended into Methanol...

27 October 2016 6,163 1 View

Does 0,1 M PBS buffer could be used for cell lysis through sonication method ?

Recently, I'm trying to lyse the bacterial pellet with sonication to get the protein. Before running the sonication, the pellet was re-suspended into 0,1M PBS buffer. After running the sonication,...

11 October 2016 9,852 6 View

I'm running MS-HRM PCR for Nasopharyngeal Cancer marker, I have an issue with melting curve of control standart ?

I'm using 100% methylation & 0% methylation plasmid for standart control. Then, we mix them to get 75%, 50%, 25% & 10% standart. Then, we convert with BIsulfite modification and run in...

06 October 2016 522 1 View

Dear, why in HPLC method with detector UV-Vis not appear peak in chromatogram in wavelength maximum?

In my research, to check purity of my synthesized compound I use HPLC method with detector UV-Vis. So, first I did spectroscopy UV my compound and appear two peaks maximum (237 & 367 nm) ....

15 May 2016 6,989 8 View

Should I re-spot the white colonies in new blue-white screening plate after first screening?

My supervisor suggested me that I should re-spot the white colonies in new plate after performing first blue-white screening. This step allows white colonies to develop the color of colonies...

07 August 2015 7,710 3 View

Why does the color of my transformant colonies turn into blue?

I already have performed the blue white screening and got the white colonies (transformants). I cultured the transformants onto blue white screening plate again (already containing X-gal,...

03 June 2015 7,215 3 View

Which agarose percent should I use to quality check genomic DNA by gel electrophoresis?

My supervisor usually use 1% agarose gel to check the DNA genomic quality of human cancer cells. But in this attached book, it is stated that it should use agarose gel with percentage less than...

18 March 2015 2,280 11 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View