Hi
Dear @all,
I want to do enzymatic activity assay of purified protein. How can I know, which inhibitor and substrate are specific for transcription activator protein? when I have analyzed the sequence via conserved domains NCBI, it shows two domains namely, autoinducer binding domain and helix-turn-helix(HTH-LuxR) domain. I have searched literature but did not find the suitable answer. Kindly help me, is there any relevant protocol or material share with me.
Thanks a lot!
Hi Farman,
You can have look here: https://www.brenda-enzymes.org/. It is a huge data base for enzymes and inhibitors.
Hi Farman,
I am not exactly sure for the specificity of my answer to this question but as I see you have HTH part. Almost all the HTH are DNA binding domains of such response regulators. Such regulators requires a divalent cation such as Mg2+, Ca2+, Zn2+ to initiate transcription. Hope it helps in shaping your experiment.
Regards,
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