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Questions related from Farman Ali
Is there any tool or program to predict the synergy of two drugs based on bioinformatics without experimental data. I've more than 30 active TCM compounds that target hypertension associated...
11 November 2019 6,233 3 View
Hi @all !!! I have used mcule data base by structure-based virtual screening aginst target receptor. I have down loaded smile file and tried to genrate mole2 file using USCF chimera...
11 November 2018 2,481 8 View
Hi every body ! lot of iron uptake mechanisms are studied in bacteria but its not well known How iron relates to Quorum sensing is there any pathway?
03 March 2018 8,699 2 View
Hi Dear @all, I want to do enzymatic activity assay of purified protein. How can I know, which inhibitor and substrate are specific for transcription activator protein? when I have analyzed...
08 August 2017 8,681 2 View
This protein is GST-Tagged and also soluble. But I'm facing problem in the purification step.PBS buffer pH7.3 as a binding buffer and Tris-HCl + L-Glutathione (reduced )pH.8. this protein eluted...
02 February 2017 8,719 4 View
Please suggest me, I have cloned some proteins by using pET32a and pGEX-KG vectors. Their overexpression and purification are good. Now i want to move towards crystallization here i have a few...
01 January 2017 9,039 4 View
Hi everyone , For example, I have a posttranslational modified protein sequence (PTM). I have drawn its 3Dmodel by using swiss model workspace and then downloaded this model and attached here for...
11 November 2016 7,464 3 View
Hi every body! i am trying to pool the protein concentration after removal of his tag from native protein for crystal but did not get required quantity. When I expressed, purified and concentrated...
03 March 2016 3,726 3 View
I have expressed a protein in E.coli BL21(DE3) expression vector system.This targeted protein always remains in sediment. For the elution of this protein I have tried many times to change the pH...
01 January 2016 7,367 4 View