Farman Ali

9 Questions 4 Answers 0 Followers

Questions related from Farman Ali

How to predict the Synergy of two drugs?

Is there any tool or program to predict the synergy of two drugs based on bioinformatics without experimental data. I've more than 30 active TCM compounds that target hypertension associated...

11 November 2019 6,235 3 View

How to convert macromolecule inhibitors from smiles format to mole2 format?

Hi @all !!! I have used mcule data base by structure-based virtual screening aginst target receptor. I have down loaded smile file and tried to genrate mole2 file using USCF chimera...

11 November 2018 2,482 8 View

How iron relates to bacterial Quorum sensing ?

Hi every body ! lot of iron uptake mechanisms are studied in bacteria but its not well known How iron relates to Quorum sensing is there any pathway?

03 March 2018 8,699 2 View

Is there any bioinformatics program to find out the specific inhibitor and substrate for enzymatic activity assay of purified protein?

Hi Dear @all, I want to do enzymatic activity assay of purified protein. How can I know, which inhibitor and substrate are specific for transcription activator protein? when I have analyzed...

08 August 2017 8,682 2 View

Hi everybody! Kindly suggest me how to purify this protein?

This protein is GST-Tagged and also soluble. But I'm facing problem in the purification step.PBS buffer pH7.3 as a binding buffer and Tris-HCl + L-Glutathione (reduced )pH.8. this protein eluted...

02 February 2017 8,720 4 View

Hi everybody! Which kit of buffers is used at the initial stage of crystallization?

Please suggest me, I have cloned some proteins by using pET32a and pGEX-KG vectors. Their overexpression and purification are good. Now i want to move towards crystallization here i have a few...

01 January 2017 9,041 4 View

How can I find out active core site and what is the effect of lysine succinylation on active coresite or vice versa?

Hi everyone , For example, I have a posttranslational modified protein sequence (PTM). I have drawn its 3Dmodel by using swiss model workspace and then downloaded this model and attached here for...

11 November 2016 7,464 3 View

How to get required protein concentration for crystals?

Hi every body! i am trying to pool the protein concentration after removal of his tag from native protein for crystal but did not get required quantity. When I expressed, purified and concentrated...

03 March 2016 3,728 3 View

How Can I purify a protein from sediment?

I have expressed a protein in E.coli BL21(DE3) expression vector system.This targeted protein always remains in sediment. For the elution of this protein I have tried many times to change the pH...

01 January 2016 7,367 4 View