Dear Anil bhai,

I could able to get a publication which deals with the P. berhei male and female gametocytes separation. Just writing the tittle of the paper here "Proteome analysis of separated male and female gaemtocytes reveals novel sex specific Plasmodium Biology". Hope this will give you some basic idea so you can try and extrapolate in P. falciparum. Will let you know if I come across any protocols which deals with the P. falciparum specifically. Hope you are doing great with your work.

Best regards

Amit

Thanks Amit,

Thank you for the reply. I shall go through this paper and get back to you.

Regards

Anil

Hi Anil,

Your best bet would be to create a new line where male and female gametocytes express different coloured proteins (e.g. GFP and RFP) under male and female specific promotors integrated in a part of genome which doesn't affect the parasite. something similar to what has been done in P.berghei (RMgm-164) (http://www.pberghei.eu/index.php?rmgm=164 ) . You can then do FACS sorting to seperate them while they are still viable. It does involve a lot of molecular work but once you have got this line, the possibilities for useful experiments are endless!

I hope that helps.

Anubhav

Thanks Anubhav for the info. I was interested in some traditional way. However, this is worth attempting. Creating transgenic P. falciparum parasite is bit tricky, but once transgenic lines are created, it would be a fool-proof method to separate them.

Thanks again

Anil