How to know about The species, sequence type and plasmid finding, by using, Kmer finder, MLST tool and plasmid finder?

Hi everyone, currently I' m doing a course "Whole-genome sequencing and its applications" from the technical university of Denmark, working on a final project So I have five unknown samples,...

01 February 2020 7,608 2 View

Is there any Kit available for the extraction of fungus DNA?

Hi, I have extracted manually DNA from fungi, which is time consuming and also required a lot of chemicals. I have isolated many fungi from glaciers, i want to extract their DNA easily, good...

11 December 2019 1,738 11 View

How to extract good quality total DNA from fungi for metagenomic purpose?

Dear all, Good day, currently I am practicing extracting total DNA using different fungi. My main purpose is to extract total DNA and send the extracted DNA for 16S Amplicon sequencing (ITS-1 and...

11 December 2019 7,424 7 View

What is the purpose of Chilled isopropenol in the extraction of DNA?

Hello Dears, i know the purpose of isopropenol in DNA extraction, collect the DNA in the form of pellet from the sample. what is the reason and logic we use chilled isopropenol in the extraction...

11 December 2019 5,929 6 View

Which is the most recent research paper that revolutionized the field of ageing research in relation to autophagy?

various reasons there is which is involve in aging such as, Mitochondrial gene expression system suppression of autophagic activity by Rubicon is a signature of aging defects in protein...

09 October 2019 2,973 1 View

When and how Rubicon functions in neurons to suppress the autophagic activity?

autophagic activity suppression by Rubicon is a signature of aging. how many time it takes in the suppression of autophagic activity by Rubicon? When Rubicon functions in neurons? How Rubicon...

09 October 2019 7,123 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View