How the year 2021 can be made good for global community?

Many steps can be taken to make the year 2021 good for humanity. For instance, if powerful and rich countries decide and spend some part of their resources for the improvement and betterment of...

30 December 2020 830 4 View

What was the most worst thing and what was the most beneficial thing that happened for global community in 2020?

Many bad and good things happened for global community in 2020. However, the present question is about one top most worst thing and one top most good thing that happened in the world in 2020 and...

30 December 2020 6,262 3 View

What can happen in the world if large scale melting of continental and alpine glaciers occurs?

Several environmental experts have warned about the global warming and its effects on different ecosystems. It may also cause large scale melting of continental and alpine glaciers. If this...

23 December 2020 4,630 3 View

Is there any relationship/correaltion between 1) elastic modulus and adhesion or 2) elastic modulus and density of solid material or polymers?

21 December 2020 1,853 3 View

What are the political factors affecting curriculum design at the primary level in Pakistan?

This Topic I chose for my Thesis in Final Year of my Master's. I am in process of it's completion but need more suggestions and comments for the Topic to elaborate.

14 December 2020 356 3 View

I am performing enzyme inhibition assay and the substrate is fluorogenic & fluorescence was measured. Now I want to calculate Vo and Vi. How can I ??

These are the per minute fluorescence observed. Please guide me how can I determine Vi, Vo and IC50 ? By slope determination or any other method? Control: 28.11 40.30 40.78 37.61...

11 December 2020 1,037 4 View

Which countries have comparatively more secure future, agricultural or industrial?

Agriculture provides for almost all the basic needs essential for survival and industries provide mostly the luxury commodities required for comfortable life. However, some countries are heavily...

07 December 2020 2,153 15 View

How many fish kinds are found in the world and how much of them are edible as human food?

About 71% of the earth's surface is covered by the water bodies which contain huge amount of creatures. Humans are commonly concerned with fish because it is consumed almost in every part of the...

06 December 2020 8,146 4 View

Is electromagnetic radiation seriously harming immune system of human body?

Presently we are living in 'electronics age'. Use of electronics has dominated every sphere of life in almost all parts of the world. All electronic appliances release electromagnetic radiation...

08 November 2020 1,558 2 View

What is the optimal dose of tamoxifen by oral gavage in adult mice?

I am using a Cre-ERT mouse model and giving mice tamoxifen by oral gavage at 8 weeks of age. 1 dose of 6 mg of tamoxifen does not generate enough Cre activity. Does anyone have any recommendations...

17 October 2020 5,034 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View