I'm doing an experiment of sperm chromatin dispersion (SCD) assay. The low melting agarose gel is needed to set and fix cells, otherwise, the dispersion or so-called halo is irregular. My question is if any substitutes for the low melting agarose gel? Because it takes time to thaw and boil the agarose gel. I was trying to use gelatin to fix the cells, unfortunately, the solidified gelatin was dissolved when conducting the protocol for SCD assay.