Filter binding assay in reverse?-Detection of proteins that get immobilized by RNA binding?

Hey, I am characterizing a competitive inhibition of a host and viral RNA biding proteins. The RNA binding is not sequence specific but dependent on secondary structure of the RNA. I am looking...

08 January 2021 6,564 3 View

Abaqus - How to use results of an axis symmetric simulation for a 3D simulation?

Hello, I simulated in Abaqus/explict a clinching process in an axis symmetric model. Now I would like to conduct a tensile shear process where I can only apply one symmetry. Transferring the...

09 September 2020 9,737 3 View

Could you suggest works dealing with the problems of contrastive theolinguistics?

I deal with the problems of contrastive theolinguistics, get acquainted with the works of colleagues available for reading. I would be grateful if you would point to scientific works in this area...

29 November 2019 3,075 3 View

Who knows differently options for stress induction?

Hi, I am a german university student (business administration and psychology) and I am going to write my bachelor thesis. I would like to research a correlation between stress and the language....

17 September 2019 4,049 3 View

How did the delta18O of the atmosphere look like in Earth's past?

Hi all, is there any information/paper if and possibly how the deltaO of the atmospheric oxygen has change in Earth's past? Thanks, Inga

29 August 2019 4,279 2 View

Statistical test for conditional regression?

Is there any statistical test or work-around to test whether the effect of variable a on c increases if b is high? All variables were asked for on a 7-point likert scale. I thought about...

17 July 2019 3,973 2 View

Isolating bacterial RNA from eukaryotic RNA (infected tissue biopsies) for qRT-PCR - Is it necessary?

Hello all, I want to perform qRT-PCR to determine the levels of bacteria in infected tissue biopsies. We do usually isolate RNA via the trizol-phenol-chlorophorm method. In these biopsies, the...

14 November 2018 3,292 4 View

Thin double-sided infrared mirror ?

I am looking for the thinnest possible infrared mirror. A thickness of about 100 micrometers would be ideal. Does anyone have an idea ? The coating should be on both sides and the mirror should be...

14 October 2018 1,546 5 View

Can anyone suggest a software for designing primers and internal oligos on bisulfite converted DNA, instead of Beacon Designer?

07 June 2018 6,063 2 View

Does s.o. have information about the household energy supply of georgia (europe)? like how much central heating or gas is used in rural areas/cities?

i am interested in knowing which kind of energy is used to which extend and in which areas of the country

28 June 2017 5,217 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View