Is there an antibody that recognises mTurquoise2 by western blotting?

01 January 2014 1 1K Report

A colleague would like to compare levels of fusion proteins (by western blotting) using the mTurquoise2 moiety present in all of them. He would need an efficient antibody as the proteins are present in relatively low copy number. Does anyone know of a commercial antibody (e.g. raised against GFP) that recognises mTurquoise2?

Rafael Malagoli Rocha

Dear Simon,

There is this antibody from MBL International: Anti-GFP mAb, catalog number: M048-3. It is a good antibody. A monoclonal one, which is good for western (hopefully it reaches the high level of sensitivity you need). It is also suitable for in situ tests if you want. All the best!

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