Phorbol esters are usually detected and quantified in plants samples making extractions and processing them by HPLC. Is there an alternative method (qualitative or quantitative) to detect them?
As you known these molecules are unstable and are susceptible to oxidation, hydrolysis, transesterification, and epimerization during isolation process .therefore, the isolation must be conducted in oxygen-free conditions '' under inert conditions argon or nitrogen flow'' you can used FT-IR with ATR unite to do qualitative or quantitative analysis under certain conditions.