For alcian blue quatification, I have found this paper helpful http://www.ncbi.nlm.nih.gov/pubmed/8512063

One thing to clarify in case the wording of the above answer isn't clear, neither Alcian blue nor DMMB will detect unsulfated molecules

Thanks a lot for the replies!!! I will try the alcian blue method first!

cheers!! :D

Hi Jose,

While not directly related to alcian blue quantification, this paper looks at GAG quantification using Safranin-O staining and compares it with few other methods: 

http://www.ncbi.nlm.nih.gov/pubmed/8894661

Another paper looking mostly at the Safranin-O quantification:

http://www.ncbi.nlm.nih.gov/pubmed/2581923

Dear Jose

I try to find a quantitative protocol for alician blue staining, It could be appropriated if you can share your experiences. Thanks a lot for your time and attention.

You have to ask one of my co- Authors

Hi Sonia,

You can use PAS Alcian Blue Stain in your tissue. Once stained you can capture representative images and use Image J to morphometry the area.

Take a look:

www.ihcworld.com/_protocols/special.../alcian_blue_pas_ellis.htm

Hi,

You can find a simple protocol in the following paper:

https://link.springer.com/article/10.2478/s11756-018-0080-z?shared-article-renderer