Is it possible to create an organoid containing stroma?

Hi, I work on mouse oviduct organoids and would like to study the stromal cells. Is it possible to create an organoid containing stroma? So far I have created organoids from mouse oviduct tissue...

04 August 2020 9,236 1 View

How many images does Imagedatagenerator generate (in deep learning)?

Can anybody explain to me this? Is there any formula or equation to predict manually, the number of images that can be generated.

19 April 2020 2,766 3 View

Can anyone address this Ansy system coupling error "Fluent threw an exception" ?

I have tried searching a lot and it keeps giving this particular error again and again. Note; The Ram and disk space are not an issue. The convergence has taken place. This error pops up once we...

17 January 2019 2,048 5 View

Why do HEK 293 cells detach as a sheet/layer after transfection?

I seeded the cells into a 12 well plate and transfected 1 ug of plasmid /well. For some reason, after 24 hours, I notice that in some wells, the cells have detached as a sheet. The entire layer of...

05 September 2017 4,034 12 View

What concentration of amino acids are required for bacterial growth in minimal media?

24 February 2016 5,534 3 View

How can we avoid agglomeration in nanoparticles?

30 March 2015 9,582 11 View

How we can isolate particularly pRET-B plasmid from the rosetta?

Master plate of DH5a is not working now, but I have that plasmid in rosetta that contains its own plasmid also. So how can I isolate and purify my own one?

07 May 2014 4,109 3 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to show arrows over the streamlines in CFD-post?

I follow this to make streamlines like the photos attached below: (software- CFD post-processing) First I plot the 2D streamline over a surface "streamline 1". them I plot a vector by the...

01 March 2021 8,668 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View