I have a suggestion but not sure it will works. Immerse the specimen into the liquid Nitrogen for some time and then grind it.

Yes I also suggest freeze and cut. The cutting/mesh handling gets easier, If you soak the mesh in e.g. cyclohexen prior to freezing. The cyclohexen will keep the mesh together and you can also freeze it on e.g. glass if you need more precise cuts.

could you specify the question a little more?

Did you though about laser cutting?

According to our experiences you can try to use an Ultraturrax in a suspending solvent. The resulting length of shredded pieces (broad distribution) depends on treatment time and rotations per minute.

Thank you all for the contribution to the topic. I am sorry, I left you without answer for that long time.

Dear Kunlin, I once tried to use liquid nitrogen for breaking the PLLA nanofibrous mat, but unfortunatelly it only swelled and did not become hard and solid. So, Marc, thank you for the suggestion about cyclohexan soaking prior freezing. I will try it as soon as possible.

Dear Helga, I considered using some homogenizer, however the one you suggested seems to be a good idea. I will try to use some similar equipment (if I will find it soon).

Dear Gopinathan, I never considered using any medical devices for this kind of nanofibrous material post-processing. I will check this equipment on line and maybe try it some day.

Thank you all for your contribution. I hope that the question will find more followers and more interesting answers.

Best regards,

Michał W.

Dear Anthimos,

I hope to find the simpliest method as posiible. The laser cutting might be very good idea, however further application of the product to the market might be too expensive.

Best regards,

Michał W.