I am wondering if anyone knows of a way to separate human cells from mouse cells in a mixed population. There are some cell surface markers specific for mouse or human cells and may perhaps be used to FACS-sort the cells, but these markers may not be expressing adequately on any cell type one wants to work with. This (mixed mouse-human cells) is particularly a problem while dealing with xenograft models. So, one general way to think forward could be density-gradient or elutriation techniques; but for these techniques to be of general applicability, one has to assume that the mouse cells differ from the human cells in cell size or volume. I am not sure if this is true. Any feedback will be greatly appreciated.
I assume you aren't able to tag the donor human cells with a fluorochrome marker?
No, Michael. Take for example, murine orthotopic xenograft models of human cancers. Suppose one intends to grow human hepatoma in mouse liver, or human GBM in mouse brain. How does one separate the human cells from the host (murine) cell populations? You do not tag the human cells in such cases.
Hi Anil, do you have a single cell solution? There are global markers for most cells of a species like CD44 or CD55. You can check about the cross specify between mouse and human. We can provide non-magnetic isolation Beads for human CD44 and CD55. This way you can isolate or deplete the cells. It is a Kit solution, you need no additional instruments. More Information to the technology you will find here: http://pluriselect.com/technology.html. In case you have more questions please feel free to contact me. Best wishes Michael
Dear Michael, thank you so much. I was thinking of coating the plate with CD44 or CD55 but shall try this kit. There would be one problem, though: there would be some tissue aggregates.
Dear Anil, coating the plate with CD44 or CD55 will work in case your cells are no adherent. Otherwise specific separation of your cells will be more efficient. To remove unwanted tissue aggregates you could use our pluriStrainer (for cell straining) with 10µm, 20µm or 27µm. More information you will find here: http://pluriselect.com/product-details/product/pluristrainer.html. I hope you will find the information helpful. Best Michael
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