Hi,

I found this, which I thought might help

Cells tracking in a live zebrafish embryo. - NCBI

https://www.ncbi.nlm.nih.gov/pubmed/18002285

All the best !

Hi,

According to the valuable answer of Suraiya, I send you the cited (free of charge) paper.

1/Please give your sentence :

live-stain the nuclei in the early zebrafish embryo

on Google or Google Scholar and you ´ll obtain a plenty of hits (see picture )

2/Concerning your sentence:

Would it be possible to stain the embryo with something like Hoechst or DAPI? And would I need to wash out the dye since I want to image the embryo over several hours?

You can try to give the Hoechst (What a kind??) or DAPI to the fishes and see later the transport via eegs/Embryos. Please note that Hoechst dyes and DAPI are cationic dyes.

Washing with what?? Water , buffer (what a kind of pH in relation to the cited dyes)?

3/On other part, please read my full text (Poster) of my former research about zebra fish embryo staining with dyestuffs (Sudan and s.o.) on RG.

The transport of such dyes is useful to understand the transport of fluorochromes during cell tracking too.

You must read a big amount of papers for a valuable research and for obtaining good and reproducible results.

Good luck

JRG

Hi,

I use Draq 5 when I wanna image live embryos in LightSheet. But the problem is time of incubation - different tissue and different stages need different time of incubation. Its not toxic for fish. You can also inject embryos with mRNA encoding GFP/RFP fused to histones. I used H2B-RFP and it work well :)

Hi Tomasz Obrębski ,

I have a few questions pertaining to Draq 5 - what concentrations did you use? How old were the embryos? And how long did you end up incubating for. I know you said it seems to vary depending on the tissue being imaged, but I figured it might give me a good frame of reference to try. Thank you!