Hello,
I'm using CMTPX cell tracker for RBC and the cells look in good shape. I'm wondering if there is a way to know if the RBC are actually alive or dead, or is it the fact that cell tracker being absorbed is a sign of being alive or viable?
Hello Mr. Zack Hi ,
1. Simply acquire sample your RBC sample in flow cytometry. Look at the FSC & SSC. Dead cells will have scattered and lowest FSC & SSC .
2. If your erythroid cell populations are immature, you can use 7AAD to distinguish between live and dead cell.
3. You can simply look at the morphology of red blood cells in microscopy to distinguish live and dead cells.
4.You also can use viability dye to distinguish live and dead cells.
Best regards,
Babu
PS exposure (Annexin V) is indicative of aged RBC. Upon PS exposure they become susceptible to phagocytosis
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