Is there a way to increase chloroplast quantity in tobacco or rather increase the size of chloroplasts?

11 April 2013 0 8K Report

I want to manufacture sesquiterpenes in the chloroplast and afterwards to increase the yield through elevating the quantity of chloroplast.

Badges
Science topic

More Shai Duchin's questions See All

Is the ICBM-DTI-81 Atlas acceptable for use?

I am interested in using this DTI atlas ( http://www.bmap.ucla.edu/portfolio/atlases/ICBM_DTI-81_Atlas/ ) but came across a publication suggesting it is not oriented properly (...

19 April 2020 6,770 4 View

How can I save multiple output files from Kubios at once?

I have 300+ participants to analyze in Kubios for heart rate variability. Currently, the only option I know of, is to save each data file one by one, which is very time consuming. Is there a way...

24 September 2017 8,707 1 View

Besides T7 RNA Polymerase, is there any other protein that binds the T7 promoter?

I know that T7 RNA polymerase does not require transcription factors , but is there any other protein besides the polymerase who can bind to the same promoter?

10 January 2017 8,049 2 View

Can anyone direct me to a good protocol and practical suggestions on how to perform loose patch?

I would like to patch neurons using this technique. I have read several protocols on how to do so, but would like more practical details...

08 September 2014 853 5 View

Can anyone please recommend me articles/sites/ppts that have an overview of cortical segmentation process?

I will be using LONI Pipeline and Brainsuite to process these.

31 October 2013 6,042 2 View

How to find an alternative stop solution or a way to counter the effect of acid on the streptavidin coating (ELISA plate)?

I'm working with a methylation enzyme that requires TFA to stop the reaction. When I tried to move the solution to an ELISA plate that has a streptavidin coating, the strong acid damaged the...

20 October 2013 2,560 4 View

What is chloroplast capture through introgression?

The nuclear data inferred Hedysarum as a non-monophyletic lineage, whereas plastid sequences supported it as a monophyletic group. One of the reasons for this is chloroplast capture through...

25 January 2021 8,559 3 View

Has somebody seen a strange pattern when transiently expressing Prot-GFP fusion in Benthamiana leaves?

Hi, Has somebody seen this strange aggregation before? I don't think they are agrobacteria since they are GFP specific (only excitable by 488 nm laser). Any input is welcomed. Thanks Alaeddine

29 October 2020 2,641 3 View

How can I calculate sample size for a cross sectional comparative study?

I'm doing a study on comparing the prevalence and severity of extrapyramidal side effects between patients with schizophrenia who smoke tobacco and who don't. How can I determine the sample sizes...

15 September 2020 4,322 3 View

Why is my protein a smear when I detect with CoIP lysis buffer plus LDS?

Hi I am trying to express my protein R in tobacco leaves and do a CoIP, but I can't detect the protein R in CoIP input. I took the same tissue and added 8M Urea plus LDS protein buffer, protein R...

31 August 2020 9,534 10 View

Which component of MPOWER toolkit has shown the highest impact in India since GATS -1?

We know there is a substantial reduction in tobacco use in India as per GATS-2. Which component of MPOWER toolkit has shown the highest impact in India since GATS -1?

25 August 2020 9,254 1 View

How to perform contigs alignment to an homologous chloroplast reference?

I recovered an N amount of putative chloroplast contigs (fasta format). I need to align them to a reference genome and there is no reference genome of my species. Fortunately, there is a reference...

20 August 2020 1,162 3 View

What are the current best options to deplete rRNA in Arabidopsis?

I am interested in mRNAs only (also the ones from chloroplast). RNA input x RNA yielded approximately? Additional Kit used to concentrate RNAs + to get rid of other RNAs (tRNAs, etc...)? Thank...

01 June 2020 1,002 1 View

Need for an appropriate journal for these keywords: Asthma, Environmental tobacco smoke, behavioral interventions?

If you know a good journal about the keywords mentioned above and the following features, please let me know. regards 1) isi - scopus or pubmed indexed 2) low impact factor 3) high acceptance...

14 May 2020 7,742 6 View

What is the best methodology to do this study?

I'm writing about a type of smokeless tobacco used in asia (nicotiana rustica linn), my purpose is to find out about heavy metals such Cd, Ni, Zn, Cu and etc in nicotiana rustica linn.

02 March 2020 4,180 2 View

If i have 200 uL of chloroplasts how would i dilute it to 1:100?

Remove 200 ml of chloroplasts, being sure to mix the suspension well before removing the sample. Dilute this suspension 1:100 with isolation buffer to make a dilute chloroplast suspension. I want...

20 November 2019 4,682 1 View