Since we work mostly with genetic models, we usually genotype our mice after ear punching them for further breeding purposes.
Recently our breeding has taken up qutie a bit resulting in a high number of samples and quite a bit of time "wasted" on something that doesn´t lead to "valuable" results but is, nonetheless, essential for following experiments and breeding.
Does any of you know of a way to facilitate and speed-up genotyping? Someting like a machine that performs the entire workflow?
Our current lysis-PCR for 4 different genes-gel loading-electrophoresis and imaging as well as annotation takes well over half a day to a day for 100 samples and is simply no longer efficient to perform.
Thank you in advance!
To enhance the efficiency of your genotyping workflow, consider adopting automated technologies that streamline various stages of the process. Automated DNA extractors, such as the Qiagen BioSprint and KingFisher Flex, can significantly reduce manual handling by automating DNA extraction and can be seamlessly integrated with automated PCR setup systems. For a more comprehensive solution, the Fluidigm Biomark HD System offers an integrated approach for high-throughput PCR amplification and analysis, minimizing the need for multiple instruments. Additionally, capillary electrophoresis systems like the Applied Biosystems SeqStudio and the QIAxcel Advanced System automate the electrophoresis and imaging steps, drastically cutting down processing time. For even greater efficiency, consider utilizing Digital Droplet PCR technology, which provides precise, absolute quantification and rare allele detection, thereby eliminating the need for post-PCR electrophoresis. Adopting these advanced technologies could significantly expedite your genotyping operations, allowing for quicker and more reliable results.
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