Is there a way a differentiate malignant B cells from normal B cells via flow?

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Is anyone aware of any Bronze Age or Neolithic pendants made of siltstone with serrated edges?

Excavation in 2007 in Northern Scotland along the East Coast revealed a domestic site containing the unbroken half (other half is missing) of a perforated circular disc measuring L 37mm x W...

27 July 2024 6,019 0 View

How to assess the viability and manage debris in fish primary cell cultures?

Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...

10 July 2024 4,918 2 View

How to generate Load deformation curve in Plaxis 3D software. After selecting curve option, box opens to select nearest points then clicked to....?

To generate curve but a straight line generated on zero load.

09 July 2024 2,617 1 View

Taking a cut line through deck build in silvaco?

Hi everyone hope your all doing good. I’ve been looping my scripts and am trying to take a cut line of the conduction band energy with respect to y for each loop. Basically, I want to take a...

03 July 2024 9,134 1 View

Research on black and ethnic minorities' detained under the mental health act ?

research on mental health and ethnicity

18 June 2024 3,958 0 View

What are these substances forming in my cell culture?

I am culturing HEK293 cells. When I plated these cells in a 96-well plate, the cells looked healthy and there were no precipitates. However, after I dosed them with the drug RITA, they developed...

30 May 2024 5,169 2 View

Does the active substance 1,3-Propanediamine-, N-(3-aminopropyl)-N-dodecyl affect the cheese culturing process?

I am looking into whether or not the biocidal active substance 1,3-Propanediamine-, N-(3-aminopropyl)-N-dodecyl (Diamine) adversely affects cheese production, by interfering with bacterial...

28 May 2024 2,881 0 View

Help! Alizarin Red not staining?

I'm trying to stain areas of dystrophic calcification in sections of FFPE perinatal brain tissue. Typical basophilic appearance on H&E but nothing with Alizarin Red (costochondral junction...

28 May 2024 4,068 2 View

Why we checking the GCA of a parent during hybridization program?

26 May 2024 590 1 View

Why Peroxide value increase in refined vegetable oil.?

at the time of filling the peroxide value is 0.5 however it increases with the passage of time. we have never faced such problem in past. please someone assist me in this regard.

22 May 2024 6,296 2 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

08 August 2024 6,242 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

Emily S J Edwards

The EBV+ ones like DBLCL, HL etc.

Thanks Sharareh

Govinda Sharma

If you know the gene usage of the BCR of the malignant clone(s), could you use an appropriate anti-VH or VL antibody to detect it? It should be the most dominant clone so, while your population wouldn't be totally pure, it would be highly enriched for the malignant clone. I suppose this only works if it hasn't undergone class switching...