How to calculate total flavenoid content?

16 August 2020 5,323 4 View

We are planned to conduct status of communicable disease among health institution in Gondar after COVID 19. what type of study design is appropriate?

anyone who had a tool to assess the above-proposed project please help us?

29 April 2020 1,477 8 View

Is coronavirus able to reinfect Survivors? Any scientific evidence, yet?

According to https://www.worldometers.info/coronavirus/, as of March 23, 2020 (3:00 PM, at GMT+3) About 87% of Corona cases have been reported to completely recover from the disease and got...

22 March 2020 2,122 7 View

How to calculate individual fatty acids in egg GC-FID analysis?

Details of how to quantify each fatty acid contribute to the total. Formula and procedures of calculation and interpretations...

01 January 2020 9,170 3 View

Does anyone know a company that manufactures a continuous pancakes, dosa or kisira making machine that can be used as such or tailermade for injera?

I was wondering if there exists a company that manufactures a continuous pancakes, dosa or kisira making machine that can be used as such or tailermade for injera, an Ethiopian sour bread? Please...

11 July 2019 3,209 3 View

Any good suggestion of materials that help learn R stat for beginners?

I have never used R stat before, because of the requirement of knowing programming language. But apparently, it is the only freely available program for statistics. I used SAS a couple of times. I...

17 April 2019 4,262 8 View

Can phosphate-solubilizing microorganisms (PSM) are available on market? is that possible to cultured them in the laboratory and with what media?

Because, P-solubilization ability of microorganisms is considered to be one of the most important traits associated with plant phosphate nutrition

07 April 2019 633 2 View

How to choose the correct POSCAR for VASP calculation?

Hi I have proposed a low energy structure of (ZnS)6 cluster using genetic algorithm software and prepared POSCAR (1 POSCAR) using VESTA for VASP calculation. But the POSCAR I obtained from...

20 March 2019 7,406 3 View

What do you do on the first day of class in a college or university?

What do you do on the first day of delivering a course in a university or college to take up your semester on the correct path, and operate your first day of a course into the best learning...

24 December 2017 1,935 3 View

How can undergraduate students get to the point where they understand something well?

Memory is one of the fundamental tools in the learning process. However, there is a significant difference between memorizing something and learning it. Then, how can undergraduate students go to...

15 November 2017 5,937 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View