19 October 2022 1 3K Report

A software or a method to find the pI of a list of peptides without typing them one by one by sequence in computer pI on ExPasy

More Tahiri Andriamanarivo's questions See All
Can fractional derivatives generalize the integer order derivative in the rigorous mathematical sense?

For fractional derivative relations when we replace the order of derivative by 1 does that give us the classical derivative relations?

29 August 2021 6,412 4 View

What information I can get from mesuring ash centent in broiler's litter ?

I mesured ash content for samples of poultry litter, I was wondering if there was a sort of correlation orany relation between ash and phosphorus or any other mineral or even other factor.

04 September 2019 5,184 7 View

What is the importance of litter quality (pH and Nitrogen quantity) for broilers?

A lot of factors can be related to litter quality such as, intestinal health, pododermatitis and growth performances. The question is how and if there was more factors which can be related to...

28 August 2019 6,091 9 View

How important the effect of substitution of growth promoters antibiotics Bacillus Subtilis as a probiotic on chicken broilers?

I'm looking for researches about using bacillus subtilis as a probiotic for chicken broilers and as an alternative for antibiotics growth promoters

22 August 2019 9,228 4 View

How to apply a heat stress for broiler chicken ?

I'm conducting an experiment using chicken broilers during the month of July and August, I was wondering if I can consider heat as a stress factor and if there was actual experiments with applied...

03 August 2019 5,816 14 View

What procedures to do if symptoms of salmonellosis were detected in chicks of 5 days?

I'm conducting an experiment on a flock of 600 chicks. Conditions of environment are controlled, feed and water are given ad libitum. Starting from the second day we noticed that there is a high...

12 July 2019 4,255 19 View

What e-learning standards are used in MOOC conception?

I am recently seeking for information about e-learning standards applied in MOOC so I can define the learner profile. If anyone has any research papers concerning this topic, i'd be so thankful if...

01 March 2016 2,612 7 View

What is the difference between MOOC and online platform ?

I try to find the specific characteristics of MOOC which distinguishes it from other online platform i.e Moodle

15 April 2015 7,751 20 View

Is there any research on learning analytics and MOOC?

I'm looking for contributions implementing learning analytics on MOOC

01 April 2015 1,424 7 View

Will washing with 1 X PBS of tissue slices decrease kinase activity? Does anyone know?

I am performing kinase activity arrays and am struggling with lots of blood in the tissue sample. I'm wondering whether anyone knows whether washing of the tissue slices will decrease the activity...

13 November 2014 592 4 View

Similar questions and discussions
How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

What strains of C57 mice can induce rheumatoid arthritis?

Recently I looked up a literature《A Glucose-6-Phosphate Isomerase Peptide Induces T and BCell-Dependent Chronic Arthritis in C57BL/10 Mice Arthritis without Reactive Oxygen Species and...

09 July 2024 6,657 0 View

I found trouble to run MD of my peptide with PTM in different force field. In Charm-GUI, it cant recognize the engineering residue. What can I do now?

I am working on my PTM project. I have some peptide. I got the result of doing MD of those peptide without PTM in different force field like Charm-GUI, Amber, Gromacs. Now I have to do MD of those...

05 July 2024 9,346 0 View

How to prepare a transparent solution of the peptide with the mixture of hydrophobic and hydrophilic amino acids ?

I am using a peptide and have faced challenges in preparing a transparent peptide solution. My goal is to prepare a peptide solution in MES buffer and then use the solution to immobilize the...

20 June 2024 8,622 5 View

How do I calculate endogenous peptide using Isotope Dilution Mass Spectrometry (IDMS)?

Dear Researchers, I'm trying to develop the quantification of endogenous peptide in tissue using IDMS as the primary method of characterization. I performed analysis for the calibration curve of...

17 June 2024 9,797 0 View

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