I have seen it done both ways in the literature and I am unsure of which (serum or plasma) to use. I would like to measure TNF-alpha, cardiac troponin T and brain natriuretic peptide. Thank you!
Hai Toni,
Serum is always preferred for marker analysis.
Factors need to be observed (as I know)
1. source of the marker
2. stability of the marker
3. anticoagulant effect on marker and
4. interfering substances from plasma/whole blood.
here WBCs are one of the sources for TNFs. When we add anticoagulant with blood it can be modulate TNF level. So serum is better choice for TNF analysis.
I think you can find the choices.
Regards,
Balaji
Thank you to you both! One of the papers I was reading speak about serum cardiac troponin levels but then they measure it in the plasma. So its quite confusing :)
Hai Toni,
There are card methods available to detect the troponin T level qualitatively by using serum. As I said, serum is generally preferred for most markers. But under certain circumference, anticoagulated samples, defibrinated blood samples also useful. So, you have to look the marker source and interference factors from blood source as well anticoagulating agents.
For troponin T, serum will work.
Regards,
Balaji
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