I am trying to isolate RNA from mouse ovarian fibroblasts, and need to do DNase I digestion, but ran out of buffer RDD so I'm looking for a replacement.
I am also thinking about making Buffer RDD by myself. But QIAGEN says "Standard DNase buffers are not compatible with on-column DNase digestion. Use of other buffers may affect the binding of RNA to the RNeasy membrane, reducing RNA yield and integrity." Therefore, I guess Buffer RDD is to some extent different from other standard buffers for DNaseI reaction.