I use the ultracentrifugation protocol to isolate exosomes from cell in culture (BV-2 microglia cell line). I was wondering if there is a viable protocol to isolate specifically microglia-derived exosomes from bloodstream (either through immune-precipitation or even FACS??)
Which marks are the best in this case? (considering that macrophages must be excluded from isolation).
Here's the current protocol guidelines.
Exosome Isolation by Ultracentrifugation and Precipitation and Techniques for Downstream Analyses - PubMed (nih.gov)
Article Exosome Isolation by Ultracentrifugation and Precipitation a...
However, there are so many variations in exosome isolation and purification techniques that Hector Peinado, a leading EV scientist, once described the process as a mess. People must standardize the isolation techniques. Although I do ultracentrifugation and precipitation, evidences suggest that ultrafiltration might maximize the yield.