Is there a protocol for Tht assay in neuronal cell lines?

04 August 2022 0 7K Report

I want to see abeta aggregation in 96 well plate format which can be quantified by Thioflavin t based fluorescence. Using 96 well plate in Tht assay is routine method but I am particulary interested to know if we can grow the cells on 96 well plate first and then give them a beta peptide treatment for some time to facilitate aggregate formation and then we monitor aggregate (amyloid) formation by using Tht dye on fluorimeter?

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