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Questions related from Alexandros Alexandratos
My project concerns analysis of the microbial community of sediments from aquaculture systems in concrete tanks supplied with fresh sea water. We followed a molecular approach through PCR cloning...
26 July 2018 1,023 2 View
I would like to find a protocol for protein extraction from intestinal content samples of fish. It would be great if I could skip the use of a commercial kit.
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I chose to study the possible interaction of two proteins in HEK cells using the proximity ligation assay. Has anyone tried something similar and have a starting protocol? Thanx a lot in advance!
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I am using the following rehydration buffer for my 2D samples. Do you think any of the components may be altered/ become unstable after long period of storage at -20oC and thus affect the...
06 December 2012 9,806 6 View
Is it possible to freeze a primary and secondary (Jackson ImmunoResearch) antibody mix several times and reuse it? Will the antibodies still retain their same affinity after a long period of time?...
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