Dear colleagues, please any have this book Machine Learning Mastery With Weka by Jason Brownlee?

a book for ML applications in WEKA

08 September 2019 6,042 1 View

Any recommendation about cybersecurity research from business perspective ?

Any recommendation about cybersecurity research from business perspective ?

07 August 2019 1,884 3 View

I discivered the right treatment for all cancer types after long study 1999-2013. I do my best but no one help me to make me help cancer's patients?

Dears, at 1999 I discovered that known herbs used for treating all cancers I studied them for four years and at 2003 I prepared unusual treatment from them, treating many diseases in amazing way I...

03 April 2019 1,607 4 View

Do you think that the doctor who writes the maximum numbers of antibiotic prescriptions has a role in emergence of antibiotic resistance?

Many bad doctors and pharmacists try to write or give the maximum number of antibiotic prescriptions for personal gain. Do you think this has a role in propagating antibiotic resistance? Regards

31 December 2018 7,590 0 View

In concrete crack modeling using Extended FEM how one can treat the existence of reinforcement rebar when the crack path intersect this rebar?

The Extended Finite Element is innovative method used for treatment the discontinuity problem that faced the FE model during cracking process. Still a problem raises when the concrete is...

06 July 2018 2,030 4 View

Hi there, I've two strains of bacteria and four different conditions. How can design the experiment by using Response Surface Methodology (RSM)?

The Response Surface Methodology, The Box-Behnken design, three replicates.

04 May 2018 9,117 3 View

I ask if it is possible to model cracking in reinforced concrete using Extended Finite Element method?

Extended Finite Element (XFE) method is a new approach used to treat the discontinuity results from concrete cracking. It is well known that XFE is applied sufficiently for crack propagation in...

01 February 2018 2,404 4 View

What is the fracture energy?

Brittle materials such as concrete fail by cracking when they are subjected to tensile stress and by crushing when they are subjected to compression. Most of theories of failure applied to theses...

01 February 2018 456 1 View

Is there any design code or code of practice regarding specially shaped reinforced concrete columns?

I am looking for a code of practice for design of reinforced concrete columns having arbitrary cross-sections such as angle, tee of cross shapes

31 December 2017 1,001 0 View

One of my papers has been removed from google scholar. what is the reason?

One of my papers is disappeared from google scholar last Wednesday. While its still published at IEEE xplore.

10 November 2017 7,236 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

No title

22 February 2021 9,932 11 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View