Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How is mathematical giftedness supported in different countries?

I hope for a global overview on mathematical giftedness and its support in school and/or on an extracurricular level. What programmes/opportunities are offered?

08 July 2020 7,124 4 View

GROMACS - how to solve problem coming out during a creation of new Force Field?

Following Gromacs Manual and other suggestions from different topics I create a molecule with Avogadro, I decided to employ oplsaa ff (I copied it in a local directory giving a new name) and I...

25 March 2020 4,438 3 View

How to create a fictitious atom in shelx?

Dear all, I am dealing with some crystal structure in which it is possible to replace Br- anions with Cl- anions. I am trying the refinement by setting the occupancy factor according to the molar...

24 February 2020 4,122 2 View

Trying to run RT-PCR for PDCD4 gene in human serum, can't get any values. Checked primers and they seem okay, any suggestions?

We are having difficulty detecting PDCD4 in serum. We have ordered an ELISA to attempt this, but have not tried it yet. Afraid something is wrong with serum.

08 January 2018 4,637 3 View

How to check for primer dimers?

I am having problems with high efficiency of my primers and one reason could be the formation of primer dimers. I dont see any big peak on the melt curve, but I dont know if this increased peak is...

19 September 2017 7,270 4 View

Checking RNA integrity for qPCR with electrophoresis but it does not show 28S/18S ~ 2. Should I extract again?

I have run RNA samples in 1% agarose gel but I cannot visually see a ratio 28S/18S ~ 2. What I might be doing wrong? Also, the samples marked with numbers, should I still consider them for qPCR?

12 September 2017 6,958 6 View

How can extract eva-copolymer from a wax blend?

Hello everybody,I must extract Ethylene Vinyl Acetate (EVA) copolymer from a mixture consisting of: EVA / Liquid Paraffin / Microcrystalline Wax. Can anybody give me some advice? Thank you

26 April 2017 8,351 3 View

Is there any phenotype database for Streptomyces?

I'm identifying my Streptomyces isolates at the specie level. I already performed all the phenotype / chemotaxonomic tests, and constructed a phylogenetic tree. However, I didn't find the...

16 April 2017 1,713 3 View

Griess Reagent usage in fibroblast cell culture?

Hello, I am trying to measure NO in cell culture (fibroblast cells). Using Griess reagent I try to follow the paper from Wang et al., 1996 which gives a basic protocol that does not differ from...

07 October 2016 8,597 1 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

How COVID-19 has affected mental health?

The pandemic has led to depression and anxiety and its negative effects shouldn't be ignored. However, it has also led some people to become more resilient! What do you think? Thank you.

21 February 2021 4,733 19 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

Where can I find raw quantitative datasets on ecosystem services?

I'm an Undergraduate student on the search for a research topic which has to involve any ecosystem service(s) and a response to environmental change. I'm looking for raw quantitative data sets (I...

19 February 2021 5,911 2 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View

Patients with chronic kidney disease could get COVID-19 vaccine?

04 February 2021 278 4 View

Can anyone help to convert ODK XLS into XML?

We want to conduct a verbal autopsy using WHO adult VA updated tools in ODK. The current version of WHO adult verbal autopsy tools is v1.5.3. I have successfully converted VA ODK-XLS v1.4 into XML...

03 February 2021 844 2 View