Hey! Dear colleagues,
I am designing my experiment. I want to know which nuclease can cut linear dsDNA but don't cut circular dsDNA?
To be more specific. I am looking for a nuclease that cut the extracted genomic DNA but don't cut the plasmid DNA.
Dear Wu,
I hope you must be knowing about Plasmid-safe ATP dependent DNAse marketed by epicentre. Micrococcal ATP dependent enzymes are also similar in function. Besides that, there are some DNA binding proteins such as recBC can be used to prevent the damage of circular DNA.
Did you try CSCl2 density gradient method?
I hope this would be of help.
You may refer to the following links:
http://www.epibio.com/docs/default-source/forum-archive/forum-02-1---dna-specific-nucleases.pdf?sfvrsn=6
http://www.epibio.com/enzymes/nucleases-glycosylases-dna-binding-proteins/dna-exonucleases/plasmid-safe-atp-dependent-dnase
http://www.pnas.org/content/70/10/2884.full.pdf
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