I am looking to see if FHL-1 exists in mouse as well. In humans there is the CFH and then a naturally occuring truncated form with just teh first 7 CCPs (FHL-1). Do mice have this truncated form as well?
I have the sequence for the precursor protein, C3, but what is the sequence of the activated C3b? After C3 is cleaved into C3b and C3a. I would like to attempt to make a construct that can...
31 December 2016 4,010 3 View
I would like to measure the function of CFH as a decay accelerator, but all the protocols I am reading are using a machine to do so. Is there a way to do it by hand? Possibly just mixing together...
01 February 2016 269 1 View
I had never heard of VBS until I was looking into how to perform a decay accelerating assay to look at CFH function. In this assay that is the buffer the reaction takes place in, as opposed to PBS...
01 February 2016 2,360 3 View
I am looking for an anti-PDGFRb human specific antibody used for IHC that will not cross react with monkey protein. All of the antibodies I find specific to human say they have not been tested for...
09 October 2015 6,846 5 View
I am looking for a kit, or well described assay to determine the functionality of complement factor H. I have seen brief protocols involving incubating different amounts of factor H with Facto I...
09 October 2015 8,887 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
How to enhanced h-index in Research Gate?
04 August 2024 3,368 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View