Lisa R. Keyes

6 Questions 6 Answers 0 Followers

Questions related from Lisa R. Keyes

Is there a mouse Factor H Like-1 (FHL-1) ortholog to the human FHL-1?

I am looking to see if FHL-1 exists in mouse as well. In humans there is the CFH and then a naturally occuring truncated form with just teh first 7 CCPs (FHL-1). Do mice have this truncated form...

05 May 2017 1,486 0 View

What is the sequence of human complement component C3b?

I have the sequence for the precursor protein, C3, but what is the sequence of the activated C3b? After C3 is cleaved into C3b and C3a. I would like to attempt to make a construct that can...

01 January 2017 3,984 3 View

How can I measure decay accelerating activity of CFH?

I would like to measure the function of CFH as a decay accelerator, but all the protocols I am reading are using a machine to do so. Is there a way to do it by hand? Possibly just mixing together...

02 February 2016 248 1 View

What is the function of veronal buffered saline (VBS) as the diluting buffer in decay accelerating assays?

I had never heard of VBS until I was looking into how to perform a decay accelerating assay to look at CFH function. In this assay that is the buffer the reaction takes place in, as opposed to PBS...

02 February 2016 2,334 3 View

Where can I find an anti-PDGFRb human specific antibody (that doesn't cross react with monkey)?

I am looking for an anti-PDGFRb human specific antibody used for IHC that will not cross react with monkey protein. All of the antibodies I find specific to human say they have not been tested for...

10 October 2015 6,821 5 View

What is the best kit or assay to detect complement factor H function?

I am looking for a kit, or well described assay to determine the functionality of complement factor H. I have seen brief protocols involving incubating different amounts of factor H with Facto I...

10 October 2015 8,866 6 View