Our institution does not have an HPLC coupled to an electrochemical detector to allow us the precise dosage of plasma and tissue cathecholamines. For this purpose, we want to know if anyone has already assayed cathecholamines with the UV-Vis spectrophotometer. Are the results obtained as precise as those obtained with the HPLC?
Thank you.
O. Adjroud.
The optical parameters and statistical comparison justify this method for application in routine drug estimation in pure and dosage forms. Also, the procedures do not involve any critical reaction conditions or tedious sample preparation steps.
Please look at the following below links which may help you in your analysis:
http://www.scielo.br/pdf/qn/v34n3/02.pdf
https://pubs.rsc.org/en/content/articlelanding/2015/AY/c5ay01353d#!divAbstract
Thanks
Use standard cathecholamines and prepare standard curve with it. Determine optical density of the standards and the unknown and extrapolate the unknown from the graph.
I thing that this method is not sensitive, sorry
best regards
jean-pierre
Yes you can determine cathecholamines by UV but only for raw materials and finish products. For the plasma level I think the method is not sensitive enough and besides there is many components in the plasma that need to be separated in order to avoid interference and to get the right quantitation. I do not think so but try to quantitated with UV at 200 or 195 nm. Maybe it works
The optical parameters and statistical comparison justify this method for application in routine drug estimation in pure and dosage forms. Also, the procedures do not involve any critical reaction conditions or tedious sample preparation steps.
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