Usually people use intracellular cesium to block potassium channels, and then add for example TEA to also block them. Is there any knowledge available which potassium channels are targeted by either one ?
Hi Friedrich,
There is an excellent website called "Guide to PHARMACOLOGY" run by IUPHAR and others: http://www.guidetopharmacology.org/
Cesium can be found here: http://www.guidetopharmacology.org/GRAC/LigandDisplayForward?tab=biology&ligandId=2356
TEA: http://www.guidetopharmacology.org/GRAC/LigandDisplayForward?ligandId=2343
Good luck with your experiments.
Best wishes, Christian
Dear Friedrich,
My personal experience is that Cs blocks different potassium channels partially from inside. For example while you can block Ih current with 3 mM external Cs, internally applied much higher Cs will not block Ih completely. Similarly internal Cs will partially block Kv channels, but to have complete block people adds TEA to their internal solution.
Best wishes, Refik
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In our experience you need both high Cs (120 mM) and 10-20 mM TEA internally to block potassium currents. However, in some cells and nerve terminals with large potassium currents this may not be enough and you need to have 4-AP or TEA outside. If you have BK channels use iberiotoxin or for SK channels use apamin.
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