I´m trying to establish a HER2-positive breast cancer model to study the immune response against it in mice, but so far have been unable to find a suitable cell line in the literature.
A list of cell lines express EGFR, HER2 and HER3 at different levels.
Hi,
Have you considered E0771 cells. They are ideal from primary tumor studies but they don't reliably metastasize like the 4T1 (HER2- negative).
Hi Simon, thanks for your quick reply. I did consider them but they are not HER2-positive. You can get stable transfectants for HER2, but I´m concerned about whether they would be a representative model for HER2-positive cancer.
Hi Raul, thanks for your answer. Unfortunately BT474 cells are of human origin and wouldn´t grow in immunocompetent mice, so I can´t use them.
Hi Vanesa,
You might consider the MC4 cell lines from C. Lanari´s Lab. Some of them express high levels of c-erbB2 (by IHC). They originated from mouse mammary adenocarcinomas growing in Balb/c mice. You can use them in syngeneic passages. One thing, they express ER and PR as well, so if your idea is to model only HER2+/ER-, this may not be a good model. This is the link to the characterization of the cell lines.
http://cancerres.aacrjournals.org/content/61/1/293.full.pdf+html
Hi Babak, thank you very much for your reply. Unfortunately all those cell lines are of human origin.
Hi Luisa, thanks so much for that! I´ll definitely look into it.
In the past I worked with H605 breast cancer cells (Hexin Chen lab, Univ of South Carolina), which are derrived from HER2/Neu Transgenic mice. These cells are HER2 positive/Overexpress HER2. There is another cell line which is developed by Massague's Lab (MSKCC) which is MMTV-Neu, from the same transgenic mice. You may use these cells only in FVBN/HER2 transgenic mice (most likely it will not grow in FVBN wild type, as HER2/Neu is Rat origin). These transgenic mouse strains have been used for about 3 decades and the biology of Breast cancer (BC) development is studied extensively.
Also there is 4T1-ERBB2 cell line (Mouse BC cells, generated by ectopic overexpression of ERBB2) avaliable from Anticancer, Inc. Although I am not sure about how much it will recapitulate the HER2 positive BC in humans, since 4T1 resembles TNBC.
Hi Vanesa, how did you go with progressing from your query two years ago? Did you find a cell line that was Her2 positive that resulted in tumours that were sensitive to anti-Her2 treatment? If so, what mouse strain did you perform the experiments in?
Thanks!
Dear Vanesa, Ii came across your post which obviously dates back. If this is still of interest to you, please contact me directly by email as i may have the line/model you are after. It is still unpublished but seems to be exactly what you are after; syngeneic, balb/c background, HER2+/ER-/PR- and metastatic.
Hi Normand,
Thank you very much for your help! I didn't really pursue that road in the end, but it's nice to know you could have helped me.
Peter, maybe you could contact Normand since he actually got to do the mouse model and he knows more than me. Good luck!
All the best!
Cheers Vanessa, it's early stages for us, I will contact Normand if we get to that point.
All the best!
Amened on 25 January 2019
I have searched for CD340 in my protein database (please see file; HepG2 fucoidan).
Only HCC tissue (with PBC) has Receptor tyrosine-protein kinase erbB-2/Erb b-2 receptor protein-tyrosine kinase/Proto-oncogene Neu/CD340 at 5.7 μg/mg tissue protein.
Other two HCC tissues (HCC tissue named as No. 6 and HepG2) have no CD340 at all.
Therefore, Receptor tyrosine-protein kinase erbB-2/Erb b-2 receptor protein-tyrosine kinase/Proto-oncogene Neu/CD340 is not linked to cancer at all.
Gene expression is under the control of virus (my unpublished estimation). HCC tissue (with PBC) uniquely has Non-structural polyprotein (Hepatitis E virus/HEV) at 7.5 μg/mg tissue protein. HCC tissue named as No. 6, Hepatoma HepG2, and other 3 liver tissues, and two cultured liver cell lines (Hc and HepG2) has no HEV. Thus, gene expression of CD340 has been induced by HEV.
By the way, humans expresses following CDs; i.e.,
CD1d, CD5 antigen-like/IgM-associated peptide, CD8-alpha, CD8-beta, CD13, CD14, CD20 antigen-like 7/Membrane-spanning 4-domains subfamily A member 10, CD21, CD29, CD30, CD40, CD44, CD45, CD49b, CD49d, CD51, CD55/Complement decay-accelerating factor, CD56, CD58, CD61, CD69, CD88, CD98, CD99, CD103/Integrin alpha-E/HML-1 antigen, CD104, CD107a, CD116/Granulocyte-macrophage colony-stimulating factor receptor alpha chain/GM-CSF-R, CD117, CD127, CD129, CD152, CD154, CD163, CD206/Macrophage mannose receptor 1, CD233/Anion exchange protein 1/Band 3 anion transport proteinSLC4A1, CD243, CD292, CD331, CD332, CD340.
By the way, species differences may be present about CD340 between humans and mouse.
