Is there a good HPLC column from Sigma for the analysis of phenolic acids?

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Mraihi Farouk

Hi Dipjyoti

I am working q lot on RP-HPLC and HPLC-ESI/MS. You can see this paper my be it can help you.

10.1016/j.arabjc.2014.11.045

Soleya Dagnon

You can see chromatograms in my publications on Purospher column-Merck and Kromasil-very good.

Thiago De Melo Silva

Hello. In this paper I could isolate in RP-HPLC five phenolic compound including phenolic acids and flavonoid. See it.

https://www.researchgate.net/publication/252985612_Effect_of_the_gamma-radiation_on_phenol_fractions_obtained_from_the_leaves_of_Echinodorus_macrophyllus_Mich

Article Effect of the γ-radiation on phenol fractions obtained from ...

Prasad Andhare

http://www.sigmaaldrich.com/catalog/product/supelco/g005719?lang=en®ion=IN

Retno Andayani Budi Muljono

I used Lichrospher 60 RP-select B for the detection of cinnamic acid and its related compounds . i.e. benzoic acid, o-coumaric acid, salicylic acid, 2,3- and 2,5 dihydroxy benzoic acid using o-anisic acid as a reference compound.