- I am looing for a RP C18 column. I would appreciate experiences from people already using the column. I will be working with plant extracts.
Hi Dipjyoti
I am working q lot on RP-HPLC and HPLC-ESI/MS. You can see this paper my be it can help you.
10.1016/j.arabjc.2014.11.045
You can see chromatograms in my publications on Purospher column-Merck and Kromasil-very good.
Hello. In this paper I could isolate in RP-HPLC five phenolic compound including phenolic acids and flavonoid. See it.
https://www.researchgate.net/publication/252985612_Effect_of_the_gamma-radiation_on_phenol_fractions_obtained_from_the_leaves_of_Echinodorus_macrophyllus_Mich
Article Effect of the γ-radiation on phenol fractions obtained from ...
http://www.sigmaaldrich.com/catalog/product/supelco/g005719?lang=en®ion=IN
I used Lichrospher 60 RP-select B for the detection of cinnamic acid and its related compounds . i.e. benzoic acid, o-coumaric acid, salicylic acid, 2,3- and 2,5 dihydroxy benzoic acid using o-anisic acid as a reference compound.
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