Hello, I am looking for an antibody which can label the lysosomes in zebrafish, nowadays I used LAMP1 from Abcam, it works nice but I cannot do a co-labeling as this antibody is extracted from rabbit, and my interested antibody, which is not a commercial antibody, also from rabbit. Therefore, if anybody know any good LAMP1 antibody from mouse or donkey? Very appriaciaed for anwsering.
If you have not already done so, it might be worth exploring http://zfin.org/ resources.
Russell Linscott Thanks a lot for your advice, I got one possible candidate for anti-LAMP1, which is #1D4B from DSHB, it might work in this case. I am going to have a try.
That is very interesting. Most sources only indicate mouse reactivity for 1D4B. I hope it works for you!
Russell Linscott Yes, I also have no confidence whether it works or not. I noticed there is one publication from eLife 2017, they used this anti-LAMP1 inzebrafish ( https://elifesciences.org/articles/22234). The price of it is relatively cheap, so I may go to have a try in this situation.
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