I want to insert two genes in a vector and transform p.aer by this vector.I want to use RBS (ribosome bind site)in order to making the vector seq shorter.Is it possible?
See if the below mentioned reference is of some help. It has helped me in another study:
Renée A. J. van Boxtel and Jos A. M. van de Klundert (1998) Expression of the Pseudomonas aeruginosa Gentamicin Resistance Gene aacC3 in Escherichia coli. Antimicrob Agents Chemother. 42(12): 3173–3178
This should work fine as long as the genes are transcribed from the same promoter and the rbs is upstream of the first gene. Once bound, the ribosome will carry on reading the mRNA until it meets a terminator or comes to the end of the molecule.