Is there a form of oxytocin which has the disulfide bond reduced to two thiols?
(Further to my earlier question, I have learnt a bit more about linear oxytocin).
This is not the linear oxytocin which has a break at Tyr-Cys but retains the disulfide bond.
If there is a linear type oxytocin with two thiols could this function like glutathione in redox reactions. Could this linear form be oxidised back to cyclic oxytocin (with a disulfide bond)?
Oxytocin is known to be an antioxidant, could this be part of the mechanism?
Can it partner with glutathione and vitamin C?
I would be grateful for any thoughts.
Phuoc-Tan
Thank you Len Leonid Mizrah,
I will have a read.
Most appreciated.
Phuoc-Tan
Dear Phuoc-Tan Diep this is definitively a very interesting technical question. Although I'm absolutely not a specialist in the field I will try to suggest to you a few relevant references. For example, please have a look at the following potentially useful articles:
On-Line Electrochemical Reduction of Disulfide Bonds: Improved FTICR-CID and -ETD Coverage of Oxytocin and Hepcidin
Article On-Line Electrochemical Reduction of Disulfide Bonds: Improv...
This article is freely available as public full text on RG.
Converting disulfide bridges in native peptides to stable methylene thioacetals
Article Converting Disulfide Bridges in Native Peptides to Stable Me...
In-Source Reduction of Disulfide-Bonded Peptides Monitored by Ion Mobility Mass Spectrometry
Article In-Source Reduction of Disulfide-Bonded Peptides Monitored b...
When you mention the antioxidant properties of oxytocin, please note that these cannot be traced back to the disulfide moiety. The disulfide unit is considered an oxidant, because it can iteslf be reduced to two –SH groups.
Good luck with your research and please stay safe and healthy! 😊
Dear Frank T. Edelmann
Thank you so much for your help.
I will have a look in these.
I like asking questions, mostly because I am curious and ignorant!
Phuoc-Tan
Dear Phuoc-Tan Diep being curious is one of the most important prerequisites for young researchers to be successful:
Nurture your scientific curiosity early in your research career
https://www.nature.com/articles/ng.2527
Your original question shows that you are certainly not ignorant! 😎
It looks like this has already been answered:
Reduction and S-nitrosation of the neuropeptide oxytocin: Implications for its biological function
- October 2007
- Nitric Oxide 17(2):82-90
- Follow journal
- DOI:
- 10.1016/j.niox.2007.06.005
- Source
- PubMed
- Jean-François Roy
- 📷Michelle N Chrétien
- 📷Barbara Woodside
- Ann M English
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- Science method