I know that this question has been out for a while, but since I don't know whether you managed to address your experimental needs I'll suggest you have a look at the linked paper:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940959/
The authors have developed a RNA purification protocol compatible with intracellular antibody staining and fluorescence-activated cell sorting (FACS), showing that the protocol itself is amenable to isolate high quality RNA suitable for RNAseq, MicroArray andd qPCR.
I hope this would help.
I made a quick screening for you, the below links may be helpful.
Article MARIS: Method for analyzing RNA following intracellular sorting
http://www.uvm.edu/medicine/vtcancercenter/?Page=facilities_flowcytometry.html&SM=facilitiessubmenu.html
PLoS One. 2007 Nov 7;2(11):e1151.
I know that this question has been out for a while, but since I don't know whether you managed to address your experimental needs I'll suggest you have a look at the linked paper:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3940959/
The authors have developed a RNA purification protocol compatible with intracellular antibody staining and fluorescence-activated cell sorting (FACS), showing that the protocol itself is amenable to isolate high quality RNA suitable for RNAseq, MicroArray andd qPCR.
I hope this would help.
Hi,
I hope that these articles will be useful. Best Luck.
https://www.nature.com/articles/s41598-018-20372-7
Article MARIS: Method for analyzing RNA following intracellular sorting
Article Fixed single-cell transcriptomic characterization of human r...
https://lists.purdue.edu/pipermail/cytometry/2010-February/038825.html
http://www.asee.org/documents/zones/zone1/2014/Student/PDFs/89.pdf
Thank You very much:
Ayoob Obaid Alfalahi
Mohammed H. Musleh
Badreddine Mechri
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