Is there a different protocol for RNA isolation from A. thaliana bolts, as opposed to leaves?

More Barry Michael Rainbird's questions See All

Can someone explain the intuition behind, Blue stars are small, but wavelength is big. Red stars are Big but their wavelength is small?

can someone use quantum mechanics to explain the intuition behind this phenomenon?

07 June 2024 9,584 2 View

Does anyone have a working copy of the HFE-Diagram macro developed by Gimenez-Forcada and Sanchez San Roman (2015) that they can share?

Does anyone have a working copy of the HFE-Diagram macro developed by Gimenez-Forcada and Sanchez San Roman (2015) that they can share? The download from the site is not functional. Thanks Barry

27 May 2024 1,908 0 View

How would i calculate the density matrix for the singluarlity at the big bang, and the entanglement entropy?

this is to see if entanglement can replace inflation and/or explain the low entropy at the beginning of the universe.

23 March 2024 4,782 4 View

Is there any evidence that the global decline in food micronutrient levels might be connected to the incidence of medical conditions such as autism?

There has been a global decline in food micronutrient levels since the 1980s. This is related to the way most crops are grown: Monocropping (predominantly corn, soybeans and wheat or rice,...

21 April 2023 1,340 15 View

Can the below mechanism generate purely electric, propellant-less propulsion?

The proposed mechanism is based on BLDC motor technology but utilizes the equal and opposite force imparted to the stator when driving the rotor at speed. Typically, the rotor performs the work,...

01 March 2023 6,258 0 View

Why are air sea fluxes of CO2 many times greater than the surface water net ecosystem production?

It is an empirical fact that CO2 air-sea flux measurements from either eddy covariance or differences in their respective partial pressures with turbulence etc are much greater than the surface...

14 December 2022 5,356 3 View

Can I measure the Ion Leakage/Conductivity of Tobacco plant leaves during Programmed Cell Death at 6hrs and 12hrs?

I need to measure the conductivity of tobacco leaves as they are undergoing PCD at timepoints of 6, 12, 18 and 24hrs. The problem that I have is if I sample the leaves at 6hrs (for example) I...

15 March 2022 9,224 0 View

Help troubleshooting failed phage transduction?

Lysate prep: donor strain is inoculated overnight in LB then diluted 1:100 into 5 mL LB + 0.2% glucose + 5 mM CaCl2, incubated for 1 hour. 100 uL of P1vir was added, then incubated until total...

23 September 2021 8,024 4 View

Help with LDH activity assay?

Is is possible to find the LDH concentration using a solution NAD+, LACATE and CAPS buffer with the LDH sample at 340nm to find the LDH concentration in human blood? what would be the protocol?

26 June 2021 6,790 2 View

Are there studies available that have looked at the minimal clinical important difference (MCID) for STAXI-2?

I am analyzing data from a pre-post study where the STAXI-2 was administered to measure changes in levels of anger. The STAXI-2 has 12 summary measures of 3 domains of anger. The "post" statistics...

09 June 2021 4,599 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View