Help with LDH activity assay?

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Adam B Shapiro

There are commercial kits for measuring LDH level in biological fluids, including blood. Here are two:

https://www.abnova.com/protocol_pdf/KA1653.pdf

https://www.abcam.com/ldh-assay-kit--lactate-dehydrogenase-assay-kit-colorimetric-ab102526.html

Farbod Bahreini

Dear Barry,

I think that you need commercial kits to meausre the concentration of LDH. The materials you have mentioned are required to measure the activity of LDH not its concentration in a sample. However, you may be able to estimate the concentration of an unknown sample if you could have LDH with known concentration and compare their activitities. On the other hand, as 340 nm is used to treace the conversion of NAD to NADH, you should make sure no ther enzyme in the assau coktail has such function.