I am planning to use Matrigel for tumor induction in mice. However on Corning I found an HC Matrigel and Growth Factor Reduced (GFR); HC Matrigel. Both are used for tumor induction in imunocompromised mice however does anyone have any experience in using either and if there are any pros and cons in either of them?
Vasanth,
If you are trying to evaluate the function of a specific growth factor,then HC(High Concentration) matrigel would not be ideal.For this you need to use the growth factor reduced matrigel.If you are looking at other cancer cell specific function,then you can use the HC matrigel.The choice is important and it is totally based on your application.
Hello Charan,
I thought that as the most probable reason. This reinforcement helps a lot. Thank you.
Beyond the growth factor supply, ECM stiffness will impact on your tumor growth, via mechanotransduction signals.
From what I remember, angiogenesis will also be disturbed with a reduced number of blood vessels (only small capillaries could "infiltrate" the tumor formed in HC).
Dr. Furlan,
Thank you very much for this information. I never thought of its angiogenesis and the ECM stiffness which could effect the growth. I will try doing some research with regards to it.
Thank you again.
Murine NSCLC cell models could become a most valuable preclinical tool, and it will be interesting to see whether these NSCLC models are also refractory to chemotherapy and/or radiation therapy as so frequently observed for NSCLC. The murine models for SCLC and especially MSCLC already have many of the features of human SCLC, including metastatic spread. One of the most important translational aspects of MSCLC will be to determine its radio- and chemosensitivity. If similar sensitivities to classical drugs like cis-platin, carboplatin, etoposide, and irinotecan are found as seen in the treatment of human SCLCs, the model might help to unravel the molecular mechanisms underlying the development of chemoresistance. Finding MSCLC relapses after therapy would be extremely important since human SCLC does invariably relapse with current tumor intervention therapies. This would make the MSCLC model invaluable for testing both classic cytotoxic therapies and targeted therapies as well as combinations thereof.
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