I want convert carboxymethyl cellulose sodium to carboxymethyl cellulose by dialysis. But I only have dialysis bags that can be used in the solution with pH=5~9.
Sorry, i do not completely understand your question. Are you saying you want to fill plastic dialysis bags with a pH solution of 1 ? I do not think any type of plastic will accept that. I will fall apart.
You will to use glass bottles.
As dialysis fluids have to be as biocompatible as they can be, their pH is as close as possible to biological circumstances, that is to say, a neutral pH.
What is the reason you want to do the conversion, can I ask ? Is it a medical experiment or scientific chemistry.
The carboxyl group on the carboxymethyl cellulose is not active. Thus, it can not react with amino group on the protein. In order to get the carboxyl group activated, I have to get the carboxyl group protonated. That why I want to do the conversion. Thank you!
There is some confusion going on here. The carboxyl groups in carboxymethyl cellulose are not from the cellulose. You usually react the cellulose OH groups with something like an acid anhydride to get the carboxyl modification.
Thanks for your answer. Reacting the cellulose OH groups with acid anhydride does yield carboxyl modification, but the number of carboxyl group is small by this way. In order to get more carboxyl groups, I want to react the OH group with sodium monochloroacetate.
You lost me. I thought you wanted to cleave ester groups on carboxymethyl cellulose to generate free -OH and -COOH.
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