Hi all. I want to set up an experiment where I add LPS to my cells and measure the cellular response to this bacterial endotoxin.
I was wondering what the correlation was between LPS amounts (ug/ml) and actual bacterial cell numbers (i.e. 10ug/ml LPS = 10^8 bacterial cells/ml)?
I realise the amount of bacterial cell wall endotoxin may vary between bacterial species but an "average" amount would be fine. I have found some data in Google books but any references etc. would be most appreciated.
I also have a second question - what amounts of LPS (ug/ml) would be considered to represent Low, Medium and High bacterial contamination?
Thanks in advance,
Gary
the ratio EPS to cell number depends on particular strain, growth medium, cultivation time not only for E.coli as Doreene showed in the publication aem00015-0212.pdf , but for many different species.
Thank you all (esp.Doreene). I do appreciate that the LPS load is strain dependent. However I am just looking for a general "rough" correlation LPS vs bacterial numbers.
That ref was a great help - thanks again,
Gary
Hi - just thought I'd add below link as its a pretty good review (includes one of the references above)
http://blog.microbiologynetwork.com/572/bacteria-and-endotoxin-concentration/