I'm tying to design an experiment wherein a starter culture of cells is treated/challenged to trigger the release of cytokines into their media. This media would then be collected and added to a pool of CD4+ Th cells to simulate their receiving the cytokine signal from the original cells, and then tracked to look at changes in their differentiation into Th1 vs. Th2 state. Does anyone know if a cell line/model system similar to this exists? Can you point me in that direction if you do?

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